P-436 Non-Essential Trace Elements in women’s biofluids are associated with worse IVF outcomes in euploid single embryo transfer cycles.

Abstract Study question What is the non-essential trace element exposure impact on reproductive outcomes of women undergoing IVF treatment? Summary answer Increased blood and urinary concentration were associated with worse outcomes. known already Lower fertility rates have been observed in recent decades industrialized regions, increasing need for assisted techniques. Factors responsible this decline human include to elements. Since these elements no biological functions, they are considered detrimental normal function organism. The most common evaluated so far those traditionally toxic, such as heavy metals metalloids (mercury, lead, cadmium arsenic), which inconsistent negative associations different treatment variables described. design, size, duration 51 who underwent an euploid single-embryo transfer (SET) after PGT-A analysis, included. Nine (barium (Ba), strontium (Sr), rubidum (Rb), arsenic (As), tin (Sn), cesium (Cs), mercury (Hg), lead (Pb), antimonium (Sb)) measured follicular fluid (FF), whole (B) urine (Urine-VOR), all collected at vaginal oocyte retrieval day (VOR), (Urine-T). These measurements correlated clinical Participants/materials, setting, methods Quantification four biofluids performed by inductively coupled plasma mass spectrometry (ICP-MS). Urine concentrations normalized creatinine, quantified Jaffe reaction. Generalized linear models employed explore ovarian response embryological (associations, estimated percentile 20th 80th increase (95% confidence intervals (CI)). Associations odds ratio (95%CI). Both unadjusted age-BMI-race-smoking-adjusted applied. Main results role chance Participants had a median age 39 years [Inter Quartile Range (IQR): 31.37, 36.50] BMI 22.97 kg/m2 [IQR: 20.63, 25.12], 50% never smoked. In adjusted models, significantly found between Hg relative frequency fertilized embryos [0.73 (0.56,0.96), p = 0.024], blastocyst arrival [0.64 (0.44,0.93), 0.019], [0.60 (0.37,0.98), 0.044]. We also urine-VOR Sn [0.72 (0.53,0.98) 0.038] [0.55 (0.39,0.77), < 0.001]. case urine-T, higher St lower number retrieved oocytes [0.71 (0.57,0.90), 0.006], mature [0.75 (0.62,0.90), 0.003], (0.62,0.83), 0.001], [0.68 (0.56,0.84), 0.001] [0.83 (0.69,1.00), 0.048]. urine-T Cs was proportion [0.65 (0.42,0.99), 0.046] while both Rb [0.066 (0.44,0.99), 0.048; 0.55 (0.34,0.89), 0.019, respectively]. Regarding outcomes, fully our suggest that As probability live birth [0.03 (0.00,0.36); goal (live birth/all participants) 0.038]. Limitations, reasons caution Further studies needed confirm association greater populations, including measurement species. Wider implications findings Exposure circulating levels significant data highlight further study identify characterize how may be affecting IVF. Trial registration not applicable